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Product Name
XRCC2 Polyclonal Antibody
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Description
Polyclonal antibody to XRCC2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
XRCC2 antibody; FANCU antibody; DNA repair protein XRCC2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human XRCC2 (NP_005422.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - XRCC2 Polyclonal Antibody
Western blot analysis of extracts of mouse testis, using XRCC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - XRCC2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human thyroid cancer using XRCC2 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - XRCC2 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using XRCC2 antibody . Blue: DAPI for nuclear staining.
Immunofluorescence - XRCC2 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using XRCC2 antibody .
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Background
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"