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Product Name
WT1 Polyclonal Antibody
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Description
Polyclonal antibody to WT1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
WT1 antibody; AWT1 antibody; EWS-WT1 antibody; GUD antibody; NPHS4 antibody; WAGR antibody; WIT-2 antibody; WT33 antibody; Wilms tumor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human WT1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - WT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using WT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Immunohistochemistry - WT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using WT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil using WT1 antibody at dilution of 1:100 (40x lens).
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Background
Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"