WNT1 Polyclonal Antibody

Cat.#: 163797

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Product Information

  • Product Name
    WNT1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to WNT1
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    WNT1 antibody; BMND16 antibody; INT1 antibody; OI15 antibody; Wnt family member 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 231-370 of human WNT1 (NP_005421.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:1000 - 1:4000
    IHC 1:50 - 1:200
  • Validations

    Western blot - WNT1 Polyclonal Antibody

    Western blot - WNT1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using WNT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human mammary cancer using WNT1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human colon carcinoma using WNT1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat liver using WNT1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat kidney using WNT1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry - WNT1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse lung using WNT1 antibody at dilution of 1:100 (40x lens).

  • Background
    Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"