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Product Name
WNT1 Polyclonal Antibody
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Description
Polyclonal antibody to WNT1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
WNT1 antibody; BMND16 antibody; INT1 antibody; OI15 antibody; Wnt family member 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 231-370 of human WNT1 (NP_005421.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:4000
IHC 1:50 - 1:200 -
Validations
Western blot - WNT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using WNT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - WNT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human mammary cancer using WNT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using WNT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using WNT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using WNT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using WNT1 antibody at dilution of 1:100 (40x lens).
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Background
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"