Product Name

WFS1 antibody

Documents

Description

WFS1 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas tissue, human heart tissue, human ovary tumor tissue. Positive IP detected in mouse brain tissue. Positive WB detected in HeLa cells, HEK-293 cells, human heart tissue, human skeletal muscle tissue, mouse brain tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 95-100 kDa

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

FLJ51211 antibody; WFRS antibody; WFS antibody; WFS1 antibody; Wolfram syndrome 1 (wolframin) antibody; WOLFRAMIN antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of WFS1 recombinant protein (Accession Number: NM_006005). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:200-1:2000

IHC: 1:50-1:500

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116824(WFS1 Antibody) at dilution of 1:600

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:116824(WFS1 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:116824(WFS1 Antibody) at dilution of 1:200 (under 40x lens).

IP Result of anti-WFS1 (IP:Catalog No:116824, 4ug; Detection:Catalog No:116824 1:600) with mouse brain tissue lysate 5200ug.

Background

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

References

Lu S, Kanekura K, Hara T. A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome. Proceedings of the National Academy of Sciences of the United States of America. 111(49):E5292-301. 2014.
Odisho T, Zhang L, Volchuk A. ATF6β regulates the Wfs1 gene and has a cell survival role in the ER stress response in pancreatic β-cells. Experimental cell research. 330(1):111-22. 2015.
Hara T, Mahadevan J, Kanekura K, Hara M, Lu S, Urano F. Calcium efflux from the endoplasmic reticulum leads to β-cell death. Endocrinology. 155(3):758-68. 2014.
Kanekura K, Ma X, Murphy JT, Zhu LJ, Diwan A, Urano F. IRE1 prevents endoplasmic reticulum membrane permeabilization and cell death under pathological conditions. Science signaling. 8(382):ra62. 2015.
Sun C, Kitamura T, Yamamoto J. Distinct speed dependence of entorhinal island and ocean cells, including respective grid cells. Proceedings of the National Academy of Sciences of the United States of America. 112(30):9466-71. 2015.
Kitamura T, Sun C, Martin J, Kitch LJ, Schnitzer MJ, Tonegawa S. Entorhinal Cortical Ocean Cells Encode Specific Contexts and Drive Context-Specific Fear Memory. Neuron. 87(6):1317-31. 2015.
Kitamura T, Pignatelli M, Suh J. Island cells control temporal association memory. Science (New York, N.Y.). 343(6173):896-901. 2014.
Unal G, Joshi A, Viney TJ, Kis V, Somogyi P. Synaptic Targets of Medial Septal Projections in the Hippocampus and Extrahippocampal Cortices of the Mouse. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35(48):15812-26. 2015.

WFS1 antibody

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