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Product Name
WDR32 antibody
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Description
WDR32 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human kidney tissue. Positive IF detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 61kd
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Tested applications
ELISA, IHC, IP, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DCAF10 antibody; FLJ23201 antibody; WD repeat domain 32 antibody; WDR32 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of WDR32 recombinant protein (Accession Number: BC003520). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116862(WDR32 antibody) at dilution of 1:800
IP Result of anti-WDR32 (IP:Catalog No:116862, 4ug; Detection:Catalog No:116862 1:300) with HepG2 cells lysate 6000ug.
Immunohistochemical of paraffin-embedded human testis using Catalog No:116862(WDR32 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:116862(WDR32 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:116862(WDR32 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
WDR32, containing seven WD repeats, is also termed DDB1- and CUL4-associated factor 10 (DCAF10), which may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (PMID: 16949367). WDR32 is able to interact with DDB1 (PMID: 16949367). WDR32 is supposed to be implicated in process of ubiquitin-dependent protein degradation system. Two isoforms are described and produced by alternative splicing. Proteintech’s WDR32 antibody 20483-1-AP majorly detects WDR32 isoform1 with a theoretical MW of 61 kDa.
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