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Product Name
VTI1B antibody
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Description
VTI1B Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, C6 cells, HeLa cells, NIH/3T3 cells. Positive IP detected in HeLa cells. Positive IHC detected in human malignant melanoma tissue. Observed molecular weight by Western-blot: 29kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
VTI1 antibody; VTI1 LIKE antibody; Vti1 rp1 antibody; VTI1B antibody; VTI1L antibody; VTI1L1 antibody; VTI2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VTI1B recombinant protein (Accession Number: NM_006370). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:116808(VTI1B antibody) at dilution of 1:500
IP Result of anti-VTI1B (IP:Catalog No:116808, 3ug; Detection:Catalog No:116808 1:1000) with HeLa cells lysate 2500ug.
Immunohistochemical of paraffin-embedded human malignant melanoma using Catalog No:116808(VTI1B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human malignant melanoma using Catalog No:116808(VTI1B antibody) at dilution of 1:100 (under 40x lens)
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Background
Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"