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Product Name
VTI1A antibody
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Description
VTI1A Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in human brain tissue, human heart tissue, human kidney tissue, human lung tissue, MCF7 cells. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 25-29kd
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MVti1 antibody; Vti1 rp2 antibody; VTI1A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VTI1A recombinant protein (Accession Number: XM_005269544). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116807(VTI1A antibody) at dilution of 1:500
Immunofluorescent analysis of NIH/3T3 cells using Catalog No:116807(VTI1A Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-VTI1A (IP:Catalog No:116807, 3ug; Detection:Catalog No:116807 1:1000) with MCF-7 cells lysate 520ug.
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Background
Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). VTI1A is involved in transport between the endosome and the trans-Golgi network. The SNARE complex of STX10, STX16, VTI1A, and VAMP3 is required for MPR (mannose 6-phosphate receptors) transport from endosomes to the Golgi after delivering lysosomal enzymes to the endocytic pathway (PMID: 18195106). Transport from early/recycling endosomes to the TGN is mediated by the SNARE complex of STX6, STX16, VTI1A and VAMP4 (PMID: 11839770; 21807881).
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References
- Laufman O, Hong W, Lev S. The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport. The Journal of cell biology. 194(3):459-72. 2011.
- Laufman O, Hong W, Lev S. The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes. Journal of cell science. 126(Pt 6):1506-16. 2013.
- Laufman O, Freeze HH, Hong W, Lev S. Deficiency of the Cog8 subunit in normal and CDG-derived cells impairs the assembly of the COG and Golgi SNARE complexes. Traffic (Copenhagen, Denmark). 14(10):1065-77. 2013.
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