-
Product Name
VPS33B antibody
- Documents
-
Description
VPS33B Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human medulloblastoma tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue. Positive WB detected in mouse testis tissue, HeLa cells, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 71 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
-
Alternative names
FLJ14848 antibody; hVPS33B antibody; VPS33B antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of VPS33B recombinant protein (Accession Number: NM_018668). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:116781(VPS33B antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human medulloblastoma using Catalog No:116781(VPS33B antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using VPS33B antibody Catalog No:116781 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
-
Background
VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).
-
References
- Cullinane AR, Straatman-Iwanowska A, Zaucker A. Mutations in VIPAR cause an arthrogryposis, renal dysfunction and cholestasis syndrome phenotype with defects in epithelial polarization. Nature genetics. 42(4):303-12. 2010.
- Wong D, Bach H, Sun J, Hmama Z, Av-Gay Y. Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification. Proceedings of the National Academy of Sciences of the United States of America. 108(48):19371-6. 2011.
- van der Kant R, Fish A, Janssen L. Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. Journal of cell science. 126(Pt 15):3462-74. 2013.
- Holme A, Hurcombe JA, Straatman-Iwanowska A, Inward CI, Gissen P, Coward RJ. Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome. Clinical kidney journal. 6(2):183-8. 2013.
- van der Kant R, Jonker CT, Wijdeven RH. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. The Journal of biological chemistry. 290(51):30280-90. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"