-
Product Name
VPS25 antibody
- Documents
-
Description
VPS25 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in HeLa cells, HepG2 cells, human brain tissue, mouse liver tissue, rat liver tissue. Observed molecular weight by Western-blot: 21 kDa
-
Tested applications
ELISA, WB, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
DERP9 antibody; EAP20 antibody; ESCRT II complex subunit VPS25 antibody; FAP20 antibody; hVps25 antibody; VPS25 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of VPS25 recombinant protein (Accession Number: NM_032353). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116776(VPS25 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116776(VPS25 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116776(VPS25 antibody) at dilution of 1:50 (under 40x lens)
-
Background
VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).
-
References
- Scheffer LL, Sreetama SC, Sharma N. Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair. Nature communications. 5:5646. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"