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Product Name
VCP antibody
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Description
VCP Mouse Monoclonal antibody. Positive WB detected in RAW 264.7 cells, SH-SY5Y cells. Positive IF detected in RAW 264.7 cells, SH-SY5Y cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 89 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
15S Mg(2+) ATPase p97 subunit antibody; IBMPFD antibody; p97 antibody; TER ATPase antibody; TERA antibody; valosin containing protein antibody; VCP antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of VCP recombinant protein (Accession Number: BC007562). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107538(VCP Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:107538(VCP Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:107538(VCP Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of RAW 264.7 cells using Catalog No:107538(VCP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
VCP(Valosin-containing protein) belongs to the AAA ATPase family.VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975).Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14)
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