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Product Name
VAPB antibody
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Description
VAPB Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue, human breast cancer tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, HeLa cells, HepG2 cells, human brain tissue, mouse brain tissue, mouse liver tissue, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 27 kDa
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Tested applications
ELISA, WB, IHC, IF, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALS8 antibody; VAMP assOCiated protein B/C antibody; VAMP B antibody; VAMP B/VAMP C antibody; VAMP C antibody; VAP B antibody; VAP B/VAP C antibody; VAP C antibody; VAPB antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VAPB recombinant protein (Accession Number: NM_004738). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunofluorescent analysis of HepG2 cells, using VAPB antibody Catalog No:116715 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-VAPB (IP:Catalog No:116715, 3ug; Detection:Catalog No:116715 1:800) with HeLa cells lysate 1840ug.
1X10^6 HepG2 cells were stained with 0.2ug VAPB antibody (Catalog No:116715, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:116715(VAPB antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:116715(VAPB antibody) at dilution of 1:100 (under 40x lens)
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116715(VAPB antibody) at dilution of 1:500
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Background
Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
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References
- McCray BA, Skordalakes E, Taylor JP. Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation. Human molecular genetics. 19(6):1033-47. 2010.
- Qiu L, Qiao T, Beers M. Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice. Molecular neurodegeneration. 8:1. 2013.
- Deidda I, Galizzi G, Passantino R. Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis. European journal of neurology. 21(3):478-85. 2014.
- Shima K, Klinger M, Schütze S. The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection. Cellular microbiology. 17(7):923-34. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"