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Product Name
VAPB antibody
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Description
VAPB Mouse Monoclonal antibody. Positive WB detected in HepG2 cells, MCF-7 cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 27 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
ALS8 antibody; VAMP assOCiated protein B/C antibody; VAMP B antibody; VAMP B/VAMP C antibody; VAMP C antibody; VAP B antibody; VAP B/VAP C antibody; VAP C antibody; VAPB antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of VAPB recombinant protein (Accession Number: NM_004738). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107536(VAPB Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107536(VAPB Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107536(VAPB Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107536(VAPB Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
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Background
Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"