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Product Name
UQCRC2 antibody
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Description
UQCRC2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive FC detected in HepG2 cells. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, human colon tissue, mouse colon tissue, mouse heart tissue, mouse placenta tissue, mouse skeletal muscle tissue, SGC-7901 cells. Observed molecular weight by Western-blot: 48kd
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Complex III subunit 2 antibody; Core protein II antibody; QCR2 antibody; UQCR2 antibody; UQCRC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UQCRC2 recombinant protein (Accession Number: NM_003366). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116636(UQCRC2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116636(UQCRC2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116636(UQCRC2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using UQCRC2 antibody Catalog No:116636 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-UQCRC2 (IP:Catalog No:116636, 4ug; Detection:Catalog No:116636 1:1000) with mouse brain tissue lysate 4000ug.
1X10^6 HepG2 cells were stained with 0.2ug UQCRC2 antibody (Catalog No:116636, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.
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