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Product Name
UPF1 antibody
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Description
UPF1 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive IF detected in HepG2 cells, MCF-7 cells. Positive WB detected in HepG2 cells, Raji cells. Positive IP detected in Raji cells. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATP dependent helicase RENT1 antibody; FLJ43809 antibody; FLJ46894 antibody; HUPF1 antibody; KIAA0221 antibody; NORF1 antibody; pNORF1 antibody; RENT1 antibody; UPF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UPF1 recombinant protein (Accession Number: NM_002911). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116625(UPF1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:116625(UPF1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:116625(UPF1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells using Catalog No:116625(UPF1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-UPF1 (IP:Catalog No:116625, 4ug; Detection:Catalog No:116625 1:500) with Raji cells lysate 2000ug.
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Background
UPF1 represents UPF1 regulator of nonsense transcripts homolog (yeast)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"