Canine IL-1R2/CD121b (Fc Tag) recombinant protein

Canine IL-1R2/CD121b (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain, is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.

Uncharacterized protein

IL1R2C; IL1R-2; IL-1RT-2; CD121 ANTIGEN-LIKE FAMILY MEMBER B; IL-1 TYPE II RECEPTOR; CDW121B; IL1RB; IL-1R-2; RATTUS NORVEGICUS; IL-1RT2; IL-1R-BETA; INTERLEUKIN-1 RECEPTOR BETA; CD121B

E2RKW1

IL1R2

Human Cells

A DNA sequence encoding the canine IL1R2 (E2RKW1) (Met1-Glu343) was expressed with the Fc region of human IgG1 at the C-terminus.

Canine

The recombinant canine IL1R2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 571 amino acids and has a predicted molecular mass of 64.8 kDa. The apparent molecular mass of the protein is approximately 65-70 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized Canine IL1R2-Fc3 at 10 μg/ml (100 μl/well) can bind biotinylated human IL1B-His (Cat:501519), The EC₅₀ of biotinylated human IL1B-His (Cat:501519) is 0.08-0.2 μg/ml.