Human IL-1R2/CD121b (His Tag) recombinant protein

Human IL-1R2/CD121b (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain, is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.

Interleukin-1 receptor type 2

CD121B; INTERLEUKIN-1 RECEPTOR BETA; IL-1R-BETA; RATTUS NORVEGICUS; IL-1RT2; IL-1R-2; CDW121B; IL1RB; CD121 ANTIGEN-LIKE FAMILY MEMBER B; IL-1 TYPE II RECEPTOR; IL-1RT-2; IL1R-2; IL1R2C

P27930

IL1R2; IL1RB

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Glu 343) of human IL1R2 (NP_004624.1) was expressed, with a fused C-terminal polyhistidine tag.

Human

The recombinant human IL1R2 consists of 342 amino acids and has a predicted molecular mass of 39.3 kDa. As a result of glycosylation, the rh IL1R2 migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.

> 98 % as determined by SDS-PAGE

Measured by its ability to inhibit IL1βdependent proliferation in D10.G4.1 mouse helper T cells.
The ED₅₀ for this effect is typically 5.5 μg/mL in the presence of 40 pg/mL of rhIL1β.