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Product Name
UHRF1 antibody
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Description
UHRF1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, MCF7 cells. Positive IF detected in HepG2 cells, Hela cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ21925 antibody; hNP95 antibody; HuNp95 antibody; ICBP90 antibody; Nuclear protein 95 antibody; RING finger protein 106 antibody; RNF106 antibody; Transcription factor ICBP90 antibody; UHRF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UHRF1 recombinant protein (Accession Number: NM_013282). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116563(UHRF1 antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using UHRF1 antibody Catalog No:116563 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-UHRF1 (IP:Catalog No:116563, 4ug; Detection:Catalog No:116563 1:1000) with HeLa cells lysate 3200ug.
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Background
UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2.
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References
- Deng W, Yan M, Yu T. Quantitative proteomic analysis of the metastasis-inhibitory mechanism of miR-193a-3p in non-small cell lung cancer. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 35(5):1677-88. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"