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Product Name
UFD1L antibody
- Documents
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Description
UFD1L Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human skeletal muscle tissue, human pancreas cancer tissue. Positive WB detected in mouse thymus tissue, HepG2 cells, Raji cells. Positive IP detected in Raji cells. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
UB recombinant protein 1 antibody; UFD1 antibody; UFD1L antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UFD1L recombinant protein (Accession Number: NM_005659). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:116551(UFD1L Antibody) at dilution of 1:1000
IP Result of anti-UFD1L (IP:Catalog No:116551, 4ug; Detection:Catalog No:116551 1:1000) with Raji cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:116551(UFD1L Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:116551(UFD1L Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:116551(UFD1L Antibody) at dilution of 1:25 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
UFD1L represents ubiquitin fusion degradation 1 like (yeast)
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References
- Olmos Y, Hodgson L, Mantell J, Verkade P, Carlton JG. ESCRT-III controls nuclear envelope reformation. Nature. 522(7555):236-9. 2015.
- Hsu CW, Yu JS, Peng PH. Secretome profiling of primary cells reveals that THBS2 is a salivary biomarker of oral cavity squamous cell carcinoma. Journal of proteome research. 13(11):4796-807. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"