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Product Name
UCP2 antibody
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Description
UCP2 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue, human hepat℃irrhosis tissue, human liver tissue, human ovary tissue, human skeletal muscle tissue. Positive WB detected in mouse skeletal muscle tissue, human skeletal muscle tissue, Jurkat cells, mouse spleen tissue, rat skeletal muscle tissue. Observed molecular weight by Western-blot: 28-30 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BMIQ4 antibody; SLC25A8 antibody; UCP 2 antibody; UCP2 antibody; UCPH antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UCP2 recombinant protein (Accession Number: NM_003355). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:116546(UCP2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116546(UCP2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116546(UCP2 antibody) at dilution of 1:50 (under 40x lens)
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Background
UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lyate be used for western blot assays.
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References
- Liu Y, Jiang H, He LY, Huang WJ, He XY, Xing FQ. Abnormal expression of uncoupling protein-2 correlates with CYP11A1 expression in polycystic ovary syndrome. Reproduction, fertility, and development. 23(4):520-6. 2011.
- Liu Y, Jiang H, Xing FQ, Huang WJ, Mao LH, He LY. Uncoupling protein 2 expression affects androgen synthesis in polycystic ovary syndrome. Endocrine. 43(3):714-23. 2013.
- Sanchez-Alvarez R, Martinez-Outschoorn UE, Lamb R. Mitochondrial dysfunction in breast cancer cells prevents tumor growth: understanding chemoprevention with metformin. Cell cycle (Georgetown, Tex.). 12(1):172-82. 2013.
- Hanaoka Y, Yasuda O, Soejima H. Tissue inhibitor of metalloproteinase-3 knockout mice exhibit enhanced energy expenditure through thermogenesis. PloS one. 9(4):e94930. 2014.
- Li P, Guo X, Lei P, Shi S, Luo S, Cheng X. PI3K/Akt/uncoupling protein 2 signaling pathway may be involved in cell senescence and apoptosis induced by angiotensin II in human vascular endothelial cells. Molecular biology reports. 41(10):6931-7. 2014.
- Xie X, Zhang J, Chen D. Effects of ghrelin on postresuscitation brain injury in a rat model of cardiac arrest. Shock (Augusta, Ga.). 43(5):490-6. 2015.
- Yu SX, Du CT, Chen W. Genipin inhibits NLRP3 and NLRC4 inflammasome activation via autophagy suppression. Scientific reports. 5:17935. 2015.
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