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Product Name
UCN2 Polyclonal Antibody
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Description
Polyclonal antibody to UCN2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
UCN2 antibody; SRP antibody; UCN-II antibody; UCNI antibody; UR antibody; URP antibody; urocortin-2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-112 of human UCN2 (NP_149976.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - UCN2 Polyclonal Antibody
Western blot analysis of extracts of rat skin, using UCN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - UCN2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using UCN2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - UCN2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using UCN2 antibody . Blue: DAPI for nuclear staining.
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Background
Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"