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Product Name
UBR2 antibody
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Description
UBR2 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas tissue. Positive IF detected in HepG2 cells. Positive WB detected in BxPC-3 cells, mouse cerebellum tissue, mouse heart tissue. Observed molecular weight by Western-blot: 60-66 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
bA49A4.1 antibody; C6orf133 antibody; dJ242G1.1 antibody; dJ392M17.3 antibody; DKFZp686C08114 antibody; KIAA0349 antibody; N recognin 2 antibody; RP3 392M17.3 antibody; UBR2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UBR2 recombinant protein (Accession Number: NM_001184801). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116663(UBR2 antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells, using UBR2 antibody Catalog No:116663 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:116663(UBR2 Antibody) at dilution of 1:50(under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:116663(UBR2 Antibody) at dilution of 1:50(under 40x lens)
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Background
UBR2(Ubiquitin-protein ligase E3-alpha-2) is also named as C6orf133, KIAA0349 and N-recognin-2. It is a component of the N-end rule pathway and recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It has four isoforms produced by alternative splicing.
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