-
Product Name
UBIAD1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to UBIAD1
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
UBIAD1 antibody; SCCD antibody; TERE1 antibody; UbiA prenyltransferase domain containing 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBIAD1 (NP_037451.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - UBIAD1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UBIAD1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunohistochemistry - UBIAD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using UBIAD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - UBIAD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using UBIAD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - UBIAD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using UBIAD1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - UBIAD1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using UBIAD1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Prenyltransferase that mediates the formation of menaquinone-4 (MK-4) and coenzyme Q10. MK-4 is a vitamin K2 isoform present at high concentrations in the brain, kidney and pancreas, and is required for endothelial cell development. Mediates the conversion of phylloquinone (PK) into MK-4, probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. Also plays a role in cardiovascular development independently of MK-4 biosynthesis, by acting as a coenzyme Q10 biosynthetic enzyme: coenzyme Q10, also named ubiquinone, plays a important antioxidant role in the cardiovascular system. Mediates biosynthesis of coenzyme Q10 in the Golgi membrane, leading to protect cardiovascular tissues from NOS3/eNOS-dependent oxidative stress.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"