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Product Name
UBE2O Polyclonal Antibody
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Description
Polyclonal antibody to UBE2O
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
UBE2O antibody; E2-230K antibody; ubiquitin conjugating enzyme E2 O antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:4000
IF 1:50 - 1:200
IP 1:50 - 1:200 -
Validations
Western blot - UBE2O Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UBE2O antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Western blot - UBE2O Polyclonal Antibody
Western blot analysis of extracts from normal (control) and UBE2O knockout HeLa cells, using UBE2O antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.CRISPR KO cell lines generated by
Immunoprecipitation - UBE2O Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug UBE2O antibody . Western blot was performed from the immunoprecipitate using UBE2O antibody at a dilition of 1:1000.
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Background
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.
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