-
Product Name
UBE2D1-Specific antibody
- Documents
-
Description
UBE2D1-Specific Rabbit Polyclonal antibody. Positive WB detected in mouse thymus tissue, HeLa cells, NIH/3T3. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 17 kDa
-
Tested applications
ELISA, WB, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
E2(17)KB1 antibody; SFT antibody; Stimulator of Fe transport antibody; UBC4/5 antibody; UBC4/5 homolog antibody; UBC5A antibody; UBCH5 antibody; UBCH5A antibody; UBE2D1 antibody; Ubiquitin carrier protein D1 antibody; Ubiquitin protein ligase D1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of UBE2D1-Specific recombinant protein (Accession Number: NM_003338). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:116518(UBE2D1 antibody) at dilution of 1:300
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116518(UBE2D1 antibody) at dilution of 1:500. (Data provided by Angran Biotech (www.miRNAlab.com)).
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:116518(UBE2D1 antibody) at dilution of 1:50 (under 10x lens)
-
Background
UBE2D1(Ubiquitin-conjugating enzyme E2 D1) is also named as SFT, UBC5A, UBCH5, UBCH5A and belongs to the ubiquitin-conjugating enzyme family. It regulates DAPK3 accumulation in promyelocytic leukemia protein nuclear body (PML-NB)by interacting with DAPK3 and stimulating its ubiquitination. It is a promiscuous E2 enzyme with an innate preference for forming polyubiquitin chains through lysine 11 (K11), lysine 48 (K48), and lysine 63 (K63) of ubiquitin(PMID:21396940). This antibody is specific to UBE2D1.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"