UBC9 antibody

Cat.#: 116509

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Product Information

  • Product Name
    UBC9 antibody
  • Documents
  • Description
    UBC9 Rabbit Polyclonal antibody. Positive WB detected in mouse spleen tissue, HEK-293 cells, human testis tissue, Jurkat cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 21kd
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    C358B7.1 antibody; UBE2I antibody; P18 antibody; SUMO conjugating enzyme UBC9 antibody; SUMO protein ligase antibody; UBC9 antibody; UBCE9 antibody; UBE2I antibody; Ubiquitin carrier protein 9 antibody; Ubiquitin carrier protein I antibody; Ubiquitin protein ligase I antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of UBC9 recombinant protein (Accession Number: NM_003345). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:116509(UBC9 antibody) at dilution of 1:50

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:116509(UBC9 antibody) at dilution of 1:50

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116509(UBC9 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116509(UBC9 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116509(UBC9 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116509(UBC9 antibody) at dilution of 1:50 (under 40x lens)

  • Background
    UBE2I is also named as UBC9, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"