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  • U2AF1 Polyclonal Antibody
  • U2AF1 Polyclonal Antibody
  • U2AF1 Polyclonal Antibody
  • U2AF1 Polyclonal Antibody

U2AF1 Polyclonal Antibody

Cat.#: 160502

Special Price 174.2 USD

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Product Information

  • Product Name
    U2AF1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to U2AF1
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    U2AF1 antibody; FP793 antibody; RN antibody; RNU2AF1 antibody; U2AF35 antibody; U2AFBP antibody; splicing factor U2AF 35 kDa subunit antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human U2AF1 (NP_006749.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:100
  • Validations

    Western blot - U2AF1 Polyclonal Antibody

    Western blot - U2AF1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using U2AF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunohistochemistry - U2AF1 Polyclonal Antibody

    Immunohistochemistry - U2AF1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat Intestine using U2AF1 antibody at dilution of 1:200 (40x lens).

    Immunohistochemistry - U2AF1 Polyclonal Antibody

    Immunohistochemistry - U2AF1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human oophoroma using U2AF1 antibody at dilution of 1:200 (40x lens).

    Immunohistochemistry - U2AF1 Polyclonal Antibody

    Immunohistochemistry - U2AF1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse liver using U2AF1 antibody at dilution of 1:200 (40x lens).

  • Background
    Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"