-
Product Name
U2AF1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to U2AF1
-
Tested applications
WB, IF
-
Species reactivity
Human
-
Alternative names
U2AF1 antibody; FP793 antibody; RN antibody; RNU2AF1 antibody; U2AF35 antibody; U2AFBP antibody; splicing factor U2AF 35 kDa subunit antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human U2AF1 (NP_006749.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - U2AF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using U2AF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - U2AF1 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using U2AF1 antibody . Blue: DAPI for nuclear staining.
-
Background
Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"