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Product Name
TWF2 Polyclonal Antibody
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Description
Polyclonal antibody to TWF2
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Tested applications
WB, IHC, IP
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Species reactivity
Human, Mouse
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Alternative names
TWF2 antibody; A6RP antibody; A6r antibody; MSTP011 antibody; PTK9L antibody; twinfilin-2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50 -
Validations
Western blot - TWF2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TWF2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.
Immunoprecipitation - TWF2 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug TWF2 antibody . Western blot was performed from the immunoprecipitate using TWF2 antibody at a dilition of 1:1000.
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Background
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"