Cynomolgus TNFSF12 (Fc Tag) recombinant protein

Cynomolgus TNFSF12 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.

Tumor necrosis factor ligand superfamily member 12 proprotein

TNFSF12; APO3L; MEMBER 12; TWEAK; TUMOR NECROSIS FACTOR (LIGAND) SUPERFAMILY; UNQ181/PRO207; TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 12; DR3L; APO3/DR3 LIGAND; APO3 LIGAND; TNF-RELATED WEAK INDUCER OF APOPTOSIS; RP23-422L16.25-001; DR3LG

F7HGN4

TNFSF12-TNFSF13; TNFSF12

Human Cells

A DNA sequence encoding the cynomolgus TNFSF12 (F7HGN4) (Ser94-His249) was expressed with the Fc region of mouse IgG1 at the N-terminus. Cynomolgus and Human TNFSF12 sequences are identical.

Cynomolgus

The recombinant cynomolgus TNFSF12 is a disulfide-linked homodimer. The reduced monomer comprises 392 amino acids and has a calculated molecular mass of 43.8 KDa. The apparent molecular mass of it is approximately 34 and 47 KDa respectively in SDS-PAGE.

(72.2+25.9) % as determined by SDS-PAGE

1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is typically 2-8 ng/mL.
2. Immobilized Cynomolgus mFc-TNFSF12 at 10 μg/ml (100 μl/well) can bind human Fc-TNFRSF12A (Cat:502881), The EC₅₀ of human Fc-TNFRSF12A (Cat:502881) is 0.07-0.15 μg/ml.