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Product Name
TNFSF12 Polyclonal Antibody
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Description
Polyclonal antibody to TNFSF12
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TNFSF12 antibody; APO3L antibody; DR3LG antibody; TNLG4A antibody; TWEAK antibody; TNF superfamily member 12 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-123 of human TNFSF12 (NP_003800.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - TNFSF12 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TNFSF12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - TNFSF12 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using TNFSF12 Antibody at dilution of 1:100 (20x lens).
Immunohistochemistry - TNFSF12 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil using TNFSF12 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TNFSF12 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using TNFSF12 Antibody at dilution of 1:100 (20x lens).
Immunohistochemistry - TNFSF12 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using TNFSF12 Antibody at dilution of 1:100 (40x lens).
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Background
Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"