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Product Name
TUFM Polyclonal Antibody
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Description
Polyclonal antibody to TUFM
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
TUFM antibody; COXPD4 antibody; EF-TuMT antibody; EFTU antibody; P43 antibody; elongation factor Tu, mitochondrial antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IP 1:50 - 1:200 -
Validations
Western blot - TUFM Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TUFM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Western blot - TUFM Polyclonal Antibody
Western blot analysis of extracts of various cells, using TUFM antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Western blot - TUFM Polyclonal Antibody
Western blot analysis of extracts of various cells, using TUFM antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - TUFM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophageal cancer using TUFM antibody at dilution of 1:100 (40x lens).
Immunofluorescence - TUFM Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using TUFM antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - TUFM Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug TUFM antibody . Western blot was performed from the immunoprecipitate using TUFM antibody at a dilition of 1:1000.
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Background
Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays also a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIG-I/DDX58-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"