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Product Name
tubulin-gamma antibody
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Description
tubulin-gamma Mouse Monoclonal antibody. Positive IF detected in MDCK cells. Positive WB detected in HepG2 cells, EC109 cells, K-562 cells. Observed molecular weight by Western-blot: 48-55 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Gamma 1 tubulin antibody; GCP 1 antibody; TUBG antibody; TUBG1 antibody; TUBGCP1 antibody; Tubulin gamma 1 chain antibody; tubulin antibody; gamma 1 antibody; tubulin-gamma antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of tubulin-gamma recombinant protein (Accession Number: NM_001070). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
Immunofluorescent analysis of (-20oc Ethanol) fixed MDCK cells using Catalog No:107651(tubulin-gamma Antibody) at dilution of 1:150 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107651(tubulin-gamma Antibody) at dilution of 1:1000
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Background
Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"