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Product Name
TTK antibody
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Description
TTK Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in MDA-MB-453s cells. Positive WB detected in MCF-7 cells, HeLa cells. Observed molecular weight by Western-blot: 97 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ESK antibody; FLJ38280 antibody; MPS1 antibody; MPS1L1 antibody; PYT antibody; TTK antibody; TTK protein kinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TTK recombinant protein (Accession Number: XM_011536099). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116479(TTK Antibody) at dilution of 1:300
IP Result of anti-TTK (IP:Catalog No:116479, 4ug; Detection:Catalog No:116479 1:300) with MDA-MB-453s cells lysate 2800ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:116479(TTK Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase(PMID:15618221). Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint(PMID:20937696). TTK mRNA is present at relatively high levels in testis and thymus, tissues which contain a large number of proliferating cells, but is not detected in most other benign tissues(PMID:1639825). It has 2 isoforms produced by alternative splicing.
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