(Trp3,Arg5)-Ghrelin (1-5) peptide

(Trp3,Arg5)-Ghrelin (1-5) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GSWFR

H-Gly-Ser-Trp-Phe-Arg-OH

5

97.9%

C₃₁H₄₁N₉O₇

651.72

Synthetic

Powder

Growth hormone secretagogue (GSH) receptor agonist based on the primary structure of ghrelin (IC50 = 10 uM). (Trp3,Arg5)-Ghrelin (1-5) (GSWFR) stimulated growth hormone secretion after intravenous or oral administration and centrally administered GSWFR increased food intake in non-fasted mice. The orexigenic effect could be inhibited by (D-Lys3)-GHRP-6 (H-3108) and also by the NPY Y1 receptor antagonist BIBO3304 suggesting that GS

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• K.Ohinata et al., Peptides, 27, 1632 (2006)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.