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Product Name
TRIP10 antibody
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Description
TRIP10 Rabbit Polyclonal antibody. Positive IF detected in BxPC-3 cells, HeLa cells. Positive WB detected in K-562 cells, BxPC-3 cells, HeLa cells, mouse skeletal muscle tissue. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 85-90 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cdc42 interacting protein 4 antibody; CIP4 antibody; HSTP antibody; Protein Felic antibody; Salt tolerant protein antibody; STOT antibody; STP antibody; TR interacting protein 10 antibody; TRIP 10 antibody; TRIP10 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIP10 recombinant protein (Accession Number: NM_004240). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:116326(TRIP10 antibody) at dilution of 1:2000
IP Result of anti-TRIP10 (IP:Catalog No:116326, 4ug; Detection:Catalog No:116326 1:1000) with K-562 cells lysate 3200ug.
Immunofluorescent analysis of BxPC-3 cells using Catalog No:116326(TRIP10 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a(CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75kD and a weaker band about 85kD.
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