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Product Name
Trefoil factor 3 antibody
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Description
Trefoil factor 3 Rabbit Polyclonal antibody. Positive WB detected in Transfected HEK-293 cells. Positive IHC detected in human colon tissue, human colon cancer tissue, human small intestine tissue.
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
HITF antibody; hP1.B antibody; Intestinal trefoil factor antibody; ITF antibody; Polypeptide P1.B antibody; TFF3 antibody; TFI antibody; Trefoil factor 3 antibody; trefoil factor 3 (intestinal) antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Trefoil factor 3 recombinant protein (Accession Number: BC017859). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116355(TFF3 Antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:116355(TFF3 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:116355(TFF3 Antibody) at dilution of 1:50
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Background
Trefoil factor 3 (TFF3) belongs to the TFF-domain peptide family which consists of three small secreted proteins (TFF1, TFF2, TFF3) that are expressed by mucous-secreting epithelia. TFF3 is a major constituent in the goblet cells in small and large intestines, especially a typical secretary peptide of the normal human antral and pyloric gastric mucosa. TFF3 is essential in regulating cell migration and maintaining normal GI mucosal integrity. TFF3 has been reported to be overexpressed at the gene and the protein level in human neoplasms such as prostate, breast, and colon cancer. TFF3 is involved in tumor cell scattering, angiogenesis, and invasion.
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References
- Miyahara N, Ishino T, Kono T. Expression of Trefoil factor family peptides in the nasal allergic mucosa. Rhinology. 50(4):408-16. 2012.
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