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Transketolase antibody

Cat.#: 116336

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Product Information

  • Product Name
    Transketolase antibody
  • Documents
  • Description
    Transketolase Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, BxPC-3 cells, HEK-293 cells, human kidney tissue, Jurkat cells, MCF7 cells, mouse brain tissue. Positive IHC detected in human lymphoma tissue, human liver tissue. Positive IF detected in BxPC-3 cells. Observed molecular weight by Western-blot: 68 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    TKT antibody; TKT1 antibody; transketolase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Transketolase recombinant protein (Accession Number: NM_001064). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116336(TKT antibody) at dilution of 1:300

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116336(TKT antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116336(TKT antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116336(TKT antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of BxPC-3 cells using Catalog No:116336(TKT Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of BxPC-3 cells using Catalog No:116336(TKT Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer’s disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.
  • References
    • Lin CC, Chen LC, Tseng VS. Malignant pleural effusion cells show aberrant glucose metabolism gene expression. The European respiratory journal. 37(6):1453-65. 2011.
    • Ricciardelli C, Lokman NA, Cheruvu S. Transketolase is upregulated in metastatic peritoneal implants and promotes ovarian cancer cell proliferation. Clinical & experimental metastasis. 32(5):441-55. 2015.
    • Xu BJ, Yan W, Jovanovic B. Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts. Proteomics. 10(13):2458-70. 2010.
    • Chen L, Shu Y, Liang X. OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin. Proceedings of the National Academy of Sciences of the United States of America. 111(27):9983-8. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"