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Product Name
Transketolase antibody
- Documents
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Description
Transketolase Mouse Monoclonal antibody. Positive IHC detected in human liver tissue, human brain tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive WB detected in human brain tissue, HeLa cells, HepG2 cells, human liver tissue, Jurkat cells. Observed molecular weight by Western-blot: 68 kDa
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
TKT antibody; TKT1 antibody; Transketolase antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Transketolase recombinant protein (Accession Number: NM_001064). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107641(TKT antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107641(TKT antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107641(TKT antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:107641(TKT Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug TKT antibody (Catalog No:107641, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
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Background
Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer’s disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"