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Product Name
Transgelin-2-specific antibody
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Description
Transgelin-2-specific Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IHC detected in human kidney tissue, human liver tissue. Positive IF detected in HepG2 cells. Positive IP detected in L02 cells. Positive WB detected in rat stomach tissue, HEK-293 cells, human stomach tissue, mouse liver tissue, mouse spleen tissue. Observed molecular weight by Western-blot: 22kd
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Tested applications
ELISA, IHC, IF, IP, FC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
TAGLN2 antibody; Transgelin 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003564). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of HepG2 cells, using TAGLN2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunohistochemical of paraffin-embedded human kidney using Catalog No:116335(Transgelin-2-specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:116335(Transgelin-2-specific antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-Transgelin-2-specific (IP:Catalog No:116335, 3ug; Detection:Catalog No:116335 1:500) with L02 cells lysate 1000ug.
rat stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:116335(Transgelin-2-specific Antibody) at dilution of 1:600
1X10^6 HepG2 cells were stained with 0.2ug Transgelin-2-specific antibody (Catalog No:116335, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
The transgelin family is a group of proteins that belong to 22kd actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.
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References
- Xiao Y, Li Y, Han J, Pan Y, Tie L, Li X. Transgelin 2 participates in lovastatin-induced anti-angiogenic effects in endothelial cells through a phosphorylated myosin light chain-related mechanism. PloS one. 7(10):e46510. 2012.
- Du YY, Zhao LM, Chen L. The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma. Journal of gastroenterology and hepatology. 31(2):384-93. 2016.
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