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Product Name
Transgelin 2 antibody
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Description
Transgelin 2 Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, HEK-293 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue, human urothelial carcinoma tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
SM22 beta antibody; SM22beta antibody; TAGLN2 antibody; Transgelin 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Transgelin 2 recombinant protein (Accession Number: NM_003564). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:116332(TAGLN2 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116332(TAGLN2 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:116332(TAGLN2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The transgelin family is a group of proteins that belong to 22kd actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 2 protein. It may cross react with other two transgelins based on the sequence similarity.
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References
- Francés R, Tumang JR, Kaku H, Gurdak SM, Rothstein TL. B-1 cells express transgelin 2: unexpected lymphocyte expression of a smooth muscle protein identified by proteomic analysis of peritoneal B-1 cells. Molecular immunology. 43(13):2124-9. 2006.
- Ladd J, Taylor AD, Piliarik M, Homola J, Jiang S. Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging. Analytical and bioanalytical chemistry. 393(4):1157-63. 2009.
- Rho JH, Roehrl MH, Wang JY. Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma. Journal of proteome research. 8(12):5610-8. 2009.
- Stephens AN, Hannan NJ, Rainczuk A. Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE. Journal of proteome research. 9(5):2438-49. 2010.
- Fukushima C, Murakami A, Yoshitomi K. Comparative proteomic profiling in squamous cell carcinoma of the uterine cervix. Proteomics. Clinical applications. 5(3-4):133-40. 2011.
- Hill JJ, Tremblay TL, Pen A. Identification of vascular breast tumor markers by laser capture microdissection and label-free LC-MS. Journal of proteome research. 10(5):2479-93. 2011.
- Elsner M, Rauser S, Maier S. MALDI imaging mass spectrometry reveals COX7A2, TAGLN2 and S100-A10 as novel prognostic markers in Barrett's adenocarcinoma. Journal of proteomics. 75(15):4693-704. 2012.
- Cristobo I, Brea D, Blanco M. Usefulness of material recovered from distal embolic protection devices after carotid angioplasty for proteomic studies. Journal of vascular and interventional radiology : JVIR. 23(6):818-24. 2012.
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