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Product Name
TPPII antibody
- Documents
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Description
TPPII Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Positive FC detected in HepG2 cells. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, K-562 cells. Observed molecular weight by Western-blot: 135-140 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ40359 antibody; TPP 2 antibody; TPP II antibody; TPP2 antibody; TPPII antibody; Tripeptidyl aminopeptidase antibody; Tripeptidyl peptidase 2 antibody; Tripeptidyl peptidase II antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TPPII recombinant protein (Accession Number: NM_003291). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116210(TPP2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116210(TPP2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116210(TPP2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TPP2 antibody Catalog No:116210 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-TPP2 (IP:Catalog No:116210, 4ug; Detection:Catalog No:107634, 1:500) with Jurkat cells lysate 2400ug.
1X10^6 HepG2 cells were stained with 0.2ug TPP2 antibody (Catalog No:116210, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).
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References
- Nahálková J. Novel protein-protein interactions of TPPII, p53, and SIRT7. Molecular and cellular biochemistry. 409(1-2):13-22. 2015.
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