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TPPII antibody

Cat.#: 107634

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Product Information

  • Product Name
    TPPII antibody
  • Documents
  • Description
    TPPII Mouse Monoclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human testis tissue. Positive IF detected in Hela cells, MCF-7 cells. Positive WB detected in Hela cells, HepG2 cells, Raji cells. Observed molecular weight by Western-blot: 135 kDa
  • Tested applications
    ELISA, WB, IHC, IF, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    FLJ40359 antibody; TPP 2 antibody; TPP2 antibody; TPPII antibody; Tripeptidyl aminopeptidase antibody; Tripeptidyl peptidase 2 antibody; tripeptidyl peptidase II antibody
  • Immunogen
    Human TPP2 Recombinant protein (His tag)
  • Isotype
    Mouse IgG2b
  • Preparation
    This antibody was obtained by immunization of TPPII recombinant protein (Accession Number: NM_003291). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107634(TPP2 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107634(TPP2 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human testis using Catalog No:107634(TPP2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:107634(TPP2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:107634(TPP2 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:107634(TPP2 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of Hela cells, using TPP2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of Hela cells, using TPP2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    1X10^6 HeLa cells were stained with 0.2ug TPP2 antibody (Catalog No:107634, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug TPP2 antibody (Catalog No:107634, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).
  • References
    • Dinter J, Gourdain P, Lai NY. Different antigen-processing activities in dendritic cells, macrophages, and monocytes lead to uneven production of HIV epitopes and affect CTL recognition. Journal of immunology (Baltimore, Md. : 1950). 193(9):4322-34. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"