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Product Name
TPM4 antibody
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Description
TPM4 Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue. Positive IP detected in mouse heart tissue. Positive IHC detected in human endometrial cancer tissue. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
TM30p1 antibody; TPM4 antibody; tropomyosin 4 antibody; Tropomyosin alpha 4 chain antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TPM4 recombinant protein (Accession Number: NM_003290). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:116207(TPM4 antibody) at dilution of 1:100
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:116207(TPM4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:116207(TPM4 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-TPM4 (IP:Catalog No:116207, 4ug; Detection:Catalog No:116207 1:300) with mouse heart tissue lysate 4000ug.
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Background
Tropomyosins are ubiquitous proteins of 30 to 45 kD associated with the actin filaments of myofibrils and stress fibers.
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References
- Bae N, Wang Y, Li L, Rayport S, Lubec G. Network of brain protein level changes in glutaminase deficient fetal mice. Journal of proteomics. 80:236-49. 2013.
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