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Product Name
TPM3 antibody
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Description
TPM3 Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue, HEK-293 cells, HeLa cells, human heart tissue, mouse heart tissue, rat heart tissue. Positive IP detected in HeLa cells. Positive IHC detected in human ovary tumor, human colon cancer, human liver cancer, human ovary. Observed molecular weight by Western-blot: 38-40kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ41118 antibody; Gamma tropomyosin antibody; hscp30 antibody; hTM5 antibody; NEM1 antibody; OK/SW cl.5 antibody; TM 5 antibody; TM3 antibody; TM30 antibody; TM30nm antibody; TPM3 antibody; TPMsk3 antibody; TRK antibody; tropomyosin 3 antibody; Tropomyosin 5 antibody; Tropomyosin alpha 3 chain antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TPM3 recombinant protein (Accession Number: NM_152263). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:116206(TPM3 antibody) at dilution of 1:800
IP Result of anti-TPM3 (IP:Catalog No:116206, 4ug; Detection:Catalog No:116206 1:300) with HeLa cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116206(TPM3 antibody) at dilution of 1:50 (under 10x lens)
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Background
Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers.
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References
- Agarwal N, Adhikari AS, Iyer SV, Hekmatdoost K, Welch DR, Iwakuma T. MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4. Oncogene. 32(4):462-70. 2013.
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