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Product Name
TPM1 Polyclonal Antibody
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Description
Polyclonal antibody to TPM1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat, Zebrafish
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Alternative names
TPM1 antibody; C15orf13 antibody; CMD1Y antibody; CMH3 antibody; HEL-S-265 antibody; HTM-alpha antibody; LVNC9 antibody; TMSA antibody; tropomyosin 1 (alpha) antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPM1 (NP_001018008.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - TPM1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TPM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - TPM1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung using TPM1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TPM1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human appendix using TPM1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TPM1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human uterus using TPM1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - TPM1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using TPM1 antibody . Blue: DAPI for nuclear staining.
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Background
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"