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Product Name
TOP2A-Specific antibody
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Description
TOP2A-Specific Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, L02 cells. Positive IP detected in HeLa cells. Positive IHC detected in human spleen tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 170-177kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
DNA topoisomerase 2 alpha antibody; TOP2 antibody; TOP2A antibody; TP2A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001067). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116247(TOP2A-Specific antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human spleen using Catalog No:116247(TOP2A-Specific antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TOP2A antibody Catalog No:116247 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TOP2A-Specific (IP:Catalog No:116247, 4ug; Detection:Catalog No:116247 1:500) with HeLa cells lysate 2000ug.
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Background
TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. The antibody is specific to the gene TOP2A.
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References
- Li X, Liu Y, Chen W. TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer. Oncotarget. 5(19):9498-513. 2014.
- Zhao W, Chen R, Zhao M, Li L, Fan L, Che XM. High glucose promotes gastric cancer chemoresistance in vivo and in vitro. Molecular medicine reports. 12(1):843-50. 2015.
- Minajigi A, Froberg JE, Wei C. Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation. Science (New York, N.Y.). 349(6245):. 2015.
- Lu C, Liu X, Liu C. Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells. Toxicological sciences : an official journal of the Society of Toxicology. 147(2):588-606. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"