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  • TOB1 antibody
  • TOB1 antibody
  • TOB1 antibody
  • TOB1 antibody
  • TOB1 antibody

TOB1 antibody

Cat.#: 116163

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Special Price 239.0 USD

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Product Information

  • Product Name
    TOB1 antibody
  • Documents
  • Description
    TOB1 Rabbit Polyclonal antibody. Positive IHC detected in human stomach tissue, human breast cancer tissue. Positive IF detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 38-45 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    APRO6 antibody; PIG49 antibody; Protein Tob1 antibody; TOB antibody; TOB1 antibody; Transducer of erbB 2 1 antibody; transducer of ERBB2 antibody; TROB antibody; TROB1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TOB1 recombinant protein (Accession Number: BC031406). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116163(TOB1 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116163(TOB1 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human stomach using Catalog No:116163(TOB1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human stomach using Catalog No:116163(TOB1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human stomach using Catalog No:116163(TOB1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human stomach using Catalog No:116163(TOB1 antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-TOB1 (IP:Catalog No:116163, 4ug; Detection:Catalog No:116163 1:1000) with HeLa cells lysate 1600ug.

    IP Result of anti-TOB1 (IP:Catalog No:116163, 4ug; Detection:Catalog No:116163 1:1000) with HeLa cells lysate 1600ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:116163(TOB1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:116163(TOB1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"