TOB1 Rabbit Polyclonal antibody. Positive IHC detected in human stomach tissue, human breast cancer tissue. Positive IF detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 38-45 kDa
ELISA, WB, IHC, IF, IP
Human,Mouse,Rat; other species not tested.
APRO6 antibody; PIG49 antibody; Protein Tob1 antibody; TOB antibody; TOB1 antibody; Transducer of erbB 2 1 antibody; transducer of ERBB2 antibody; TROB antibody; TROB1 antibody
This antibody was obtained by immunization of TOB1 recombinant protein (Accession Number: BC031406). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116163(TOB1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human stomach using Catalog No:116163(TOB1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach using Catalog No:116163(TOB1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-TOB1 (IP:Catalog No:116163, 4ug; Detection:Catalog No:116163 1:1000) with HeLa cells lysate 1600ug.
Immunofluorescent analysis of HeLa cells using Catalog No:116163(TOB1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.
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