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Product Name
TMOD4 antibody
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Description
TMOD4 Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue, HeLa cells, human brain tissue. Positive IP detected in mouse skeletal muscle tissue. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 39kd
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp779I0852 antibody; SK TMOD antibody; Skeletal muscle tropomodulin antibody; TMOD4 antibody; Tropomodulin 4 antibody; tropomodulin 4 (muscle) antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TMOD4 recombinant protein (Accession Number: XM_017001089). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:116272(TMOD4 Antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116272(TMOD4 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-TMOD4 (IP:Catalog No:116272, 4ug; Detection:Catalog No:116272 1:600) with mouse skeletal muscle tissue lysate 3600ug.
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Background
TMOD4 is a member of tropomodulin (Tmod) family proteins which are capping proteins binding to the pointed end of actin filaments and preventing both elongation and depolymerization. Four mammalian Tmod genes have been identified to date. Tomd1 is expressed in terminally-differentiated post-mitotic cells, like heart, brain, lens, vasculature, and erythrocytes; Tmod2 expressed in neurons; Tmod3 expressed ubiquitously; Tmod4 expressed only in skeletal muscles. This antibody was raised against the full-length human TMOD4 and may cross-react with other Tmods.
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References
- Bao Y, Kake T, Hanashima A, Nomiya Y, Kubokawa K, Kimura S. Actin capping proteins, CapZ (β-actinin) and tropomodulin in amphioxus striated muscle. Gene. 510(1):78-86. 2012.
- Telfer WR, Nelson DD, Waugh T, Brooks SV, Dowling JJ. Neb: a zebrafish model of nemaline myopathy due to nebulin mutation. Disease models & mechanisms. 5(3):389-96. 2012.
- Greaser ML, Pleitner JM. Titin isoform size is not correlated with thin filament length in rat skeletal muscle. Frontiers in physiology. 5:35. 2014.
- Berger J, Tarakci H, Berger S. Loss of Tropomodulin4 in the zebrafish mutant träge causes cytoplasmic rod formation and muscle weakness reminiscent of nemaline myopathy. Disease models & mechanisms. 7(12):1407-15. 2014.
- Nworu CU, Kraft R, Schnurr DC, Gregorio CC, Krieg PA. Leiomodin 3 and tropomodulin 4 have overlapping functions during skeletal myofibrillogenesis. Journal of cell science. 128(2):239-50. 2015.
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