TMEM27 antibody

Cat.#: 116182

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Product Information

  • Product Name
    TMEM27 antibody
  • Documents
  • Description
    TMEM27 Rabbit Polyclonal antibody. Positive WB detected in mouse pancreas tissue, mouse kidney tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 32kd
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    Collectrin antibody; NX 17 antibody; NX17 antibody; TMEM27 antibody; transmembrane protein 27 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TMEM27 recombinant protein (Accession Number: NM_020665). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:116182(TMEM27 antibody) at dilution of 1:500

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:116182(TMEM27 antibody) at dilution of 1:500

    Immunofluorescent analysis of HepG2 cells, using TMEM27 antibody Catalog No:116182 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using TMEM27 antibody Catalog No:116182 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Background
    TMEM27(Transmembrane protein 27) is also named as collectrin and Belongs to the TMEM27 family .It is a stimulator of beta cell replicationa and a membrane protein cleaved and shed by pancreatic beta cells that has been proposed as a beta cell mass biomarker(PMID:20386877).Western blot analysis of mouse collecting duct cell lines detected Tmem27 at an apparent molecular mass of 32 kD(11278314).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"