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Product Name
Human TFPI (His Tag) recombinant protein
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Description
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
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Protein name
Tissue factor pathway inhibitor
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Protein short names
AW552122; EPI; TFI; A630013F22RIK; TFPI; LACI; TFPI1; RP23-202K13.2
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Uniprot ID
P10646
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Gene Name
TFPI; LACI; TFPI1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) was expressed with a C-terminal polyhistidine tag.
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Protein Species
Human
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Molecular weight
The secreted recombinant human TFPI comprises 265 amino acids with a predicted molecular mass of 30.6 kDa. As a result of glycosylation, the apparent molecular mass of rhTFPI is approximately 42-45 kDa in SDS-PAGE under reducing conditions.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114). The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.
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Validations
Human TFPI Protein (His Tag) SDS-PAGE
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